Screening of solvent extracts of catharanthus roseus l. for isolation of anti-inflammatory compound

Abstract

Catharanthus roseus (L.) which is an important medicinal plant of the family Apocynaceae is used to treat many of the fatal diseases. C. roseus also possess good antioxidant potential. Catharanthus roseus, is a potent medicinal plant many of the pharmacological actions such as antimicrobial, antioxidant, anthelmintic, antifeedant, antisterility, antidiarrheal, antidiabetic effect etc. That is used to treat many of the fatal diseases. In the present study, the anti-inflammatory potential of solvent extracts of whole plant of Catharanthus roseus and isolation of anti-inflammatory compound from potent extracts was determined. Aqueous and Ethanolic solvent extracts of Catharanthus roseus were found to have significant anti-inflammatory activity at doses 250 and 300 mg/Kg during in vitro anti-inflammatory assay. During in vivo anti-inflammatory activity, the paw edema was reduced significantly in carrageenan induced albino rats through introduction of ethanolic extracts and aqueous extracts at a dosage 300 mg/Kg. Ethanolic and aqueous extracts showed potent anti-inflammatory activity in comparison to non polar solvent extracts. The extracts showed higher anti-inflammatory potential as the dose varies. Thus results showed that extracts showed significant anti-inflammatory activity in dose-dependent manner. The potent polar ethanolic extract of the plant was screened via combination of Column-TLC along with other chromatographic and spectroscopic techniques. The compound was isolated in the form of Quercetin as determined by UV light source at 254 nm on TLC glass plate.

Key words: Catharanthus roseus, solvent extracts, Quercetin, anti-inflammatory activity.