Wound healing activity of the ethanolic extract of Solanum spirale leaves indigenous to north east India

Abstract

As a part of present work objective are to investigate indigenous plant used in wound healing in northeast India, we hereby reported our findings related to wound healing activities of plant Solanum spirale (Solanaceae) leaves from in vitro and in vivo model in rats following topical application studies. The ethanolic extracts of the leaves of Solanum spirale was tested for phytochemical constituents and wound healing activity against rats. The important secondary metabolites alkaloid, glycosides, saponins, flavonoids, carbohydrates, tannins, polyphenolic compounds, protein, and fats were identified in extracts. The animals were divided into three groups with six rats in each group. Topically applied 10% w/v of plant Solanum spirale leaves extracts in saline taking 0.2% w/w nitrofurazone, ointment as standard. The results showed that ethanolic extract of Solanum spirale leaves on topical application was reduced the epithelization period from 26.12±0.73to 20.47±0.63 days control and ethanol extract respectively along with a marked decrease in the scar area from 49.42±0.83 to 35.12±0.84 mm² control and ethanol extract respectively. Significant increase in tensile strength and hydroxyproline content of plant extract were also observed and compared to the control and silver sulphadiazine. The above result revealed that the plant has remarkable wound healing potency and appear to justify the traditional use of Solanum spirale in wound healing in India and offer a scientific support to the treatment of traditional healers.

Keywords: Excision; Incision; Solanum spirale; Wound healing activity